Role of Fenugreek Seeds on both Ovarian and Vaginal Cytology on Rat's Reproductive System: Histological Review

Main Article Content

Naser A. ElSawy


Background: Reports in the published literature indicate that fenugreek seeds possess significant medicinal effects. The present review showed that fenugreek (Trigonella Foenum-graecum L) seeds contain rich number of steroids and have an estrogen and progestogen like effect.

Objective: Objective of this review was to evaluate the effects of fenugreek seeds on rat’s reproductive system.

Conclusion: This review revealed that that fenugreek seeds contain estrogen like action.

Fenugreek seeds, vaginal cytology, ovary, histology, hormones

Article Details

How to Cite
ElSawy, N. A. (2019). Role of Fenugreek Seeds on both Ovarian and Vaginal Cytology on Rat’s Reproductive System: Histological Review. Asian Journal of Research and Reports in Urology, 2(1), 1-11. Retrieved from
Review Article


Yadav R, Kaushik R, Gupta D. The health benefits of Trigonella foenum-graecum: A review. International Journal of Engineering Research and Applications. 2011;1(1):032-035.

Yadav UC, Baquer NZ. Pharmacological effects of Trigonella foenum-graecum L. in health and disease. Pharmaceutical Biology. 2014;52(2):243-254.
DOI: 10.3109/13880209.2013.826247.

Sakran AME, Faragalla HEH, Ibrahim AY, Kumar Chhotalal Mithani A, khalafalla AE. Effect of fenugreek seeds on rat's ovary: histological study. International Journal of Anatomy and Research. 2016;4(1):1945-1953.
DOI: 10.16965/ijar.2016.118

Sakran AME, Ibrahim AY. Effect of Fenugreek Seeds on Rat's vagina; Cytological Study. International Journal of Recent Scientific Research. 2015;6(10): 6871-6875.

Neelakantan N, Narayanan M, de Souza RJ, van Dam RM. Effect of fenugreek (Trigonella foenum-graecumL.) intake on glycemia: a meta-analysis of clinical trials. Nutrition Journal. 2014;13(1):7.
DOI: 10.1186/1475-2891-13-.

Younesy S, Amiraliakbari S, Esmaeili S, Alavimajd H, Nouraei S. Effects of fenugreek seed on the severity and systemic symptoms of dysmenorrhea. Journal of Reproduction & Infertility. 2014; 15(1):41-8.

Hassan AM, Khalil WKB, Ahmed KA. Genetic and histopathology studies on mice: effect of fenugreek oil on the efficiency of ovarian and liver tissues. African Journal of Biotechnology. 2016; 5(5):477-483.

Muraki E, Chiba H, Taketani K, Hoshino S, Tsuge N, Tsunoda N, Kasono K. Fenugreek with reduced bitterness prevents diet-induced metabolic disorders in rats. Lipids in Health and Disease. 2012;11:58.
DOI: 10.1186/1476-511X-11-58

Sinha R, Rauniar GP, Panday D, Adhikari S. Fnugreek: Pharmacological actions. World Journal of Pharmacy and Pharmaceutical Sciences. 2015;5(1):1481-1489.

Weinbauer GF, Niehoff M, Niehaus M, Srivastav S, Fuchs A, van Esch E, Cline JM. Physiology and Endocrinology of the Ovarian Cycle in Macaques. Toxicologic Pathology. 2008;36(7S):7S–23S.
DOI: 10.1177/0192623308327412. Fenugreek: Archeology, properties, constituents and biochemicals. [Online]; 2015.
[Accessed: 19 mar 2017]. Genotoxicity testing of a fenugreek extract [Online]; 2004. Available: S0278691504002030 [Accessed: 1 mar 2017]

Gong J, Dong H, Jiang SJ, Wang DK, Fang K, Yang DS, Zou X, Xu LJ, Wang KF, Lu FE. Fenugreek lactone attenuates palmitate-induced apoptosis and dysfunction in pancreatic β-cells. World Journal of Gastroenterology. 2015;21(48): 13457–13465.
DOI: 10.3748/wjg.v21.i48.13457.

Sauvaire Y, Petit P, Broca C, Manteghetti M, Baissac Y, Fernandez-Alvarez J, Gross R, Roye M, Leconte A, Gomis R, Ribes G 4-Hydroxyisoleucine: A novel amino acid potentiator of insulin secretion. Diabetes. 1998;47(2):206-210.

Sauvaire Y, Ribes G, Baccou JC, Loubatieères-Mariani MM. Implication of steroid saponins and sapogenins in the hypocholesterolemic effect of fenugreek. Lipids. 1991;26(3):191-197.

Sidhu GS, Oakenfull DG. A mechanism for the hypocholesterolaemic activity of saponins. British Journal of Nutrition. 1986;55(3):643-649.

Stark A, Madar Z. The effect of an ethanol extract derived from fenugreek (Trigonella foenumgraecum) on bile acid absorption and cholesterol levels in rats. British Journal of Nutrition. 1993;69(1):277-287.

Vijayakumar MV, Singh S, Chhipa RR, Bhat MK. The hypoglycaemic activity of fenugreek seed extract is mediated through the stimulation of an insulin-signaling pathway. British Journal of Pharmacology. 2005;146:41-48.

Mohammad S, Taha A, Akhtar K, Bamezai RN, Baquer NZ. In vivo effect of Trigonella foenumgraecum on the expression of pyruvate kinase, phosphoenolpyruvate carboxykinase and distribution of glucose transporter (GLUT4) in alloxan-diabetic rats. Canadian Journal of Physiology and Pharmacology. 2006;84(6):647–654.

Kumar GS, Shetty AK, Salimath PV. Modulatory effect of fenugreek seed mucilage and spent turmeric on intestinal and renal disaccharidases in streptozotocin induced diabetic rats. Plant Foods for Human Nutrition. 2005;60(2): 87–91.

Ajabnoor MA, Tilmisany AK. Effect of Trigonella foenumgraceum on blood glucose levels in normal and alloxan-diabetic mice. Journal of Ethnopharmacology. 1988;22(1):45-49.

Amin R, Abdul-Ghani AS, Suleiman MS. Effect of Trigonella foenumgraecum on intestinal absorption. Diabetes. 1987;36(1): 211A.

Hamden K, Jaouadi B, Carreau S, Aouidet A, El-Fazaa S, Gharbi N, Elfeki A. Potential protective effect on key steroidogenesis and metabolic enzymes and sperm abnormalities by fenugreek steroids in testis and epididymis of surviving diabetic rats. Archives of Physiology and Biochemistry. 2010;116(3):146–155.

Kawabata T, Cui MY, Hasegawa T, Takano F, Ohta T. Anti-inflammatory and anti-melanogenic steroidal saponin glycosides from Fenugreek (Trigonella foenumgraecum L.) seeds. Planta Medica. 2011;77(7):705-710.

Sumanth M, Kapil P, Mihir P. Screening of aqueous extract of Trigonella foenumgraecum seeds for its antiulcer activity. International Journal of Research in Pharmaceutical and Biomedical Sciences. 2011;2(3):1085-1089.

Ahmadiani A, Javan M, Semnanian S, Barat E, Kamalinejad M. Anti-inflammatory and antipyretic effects of Trigonella foenum-graecum leaves extract in the rat. Journal of Ethno Pharmacology. 2001;75(2-3):283-286.
DOI: 10.1016/s0378-8741(01)00187-8

Raju J, Patlolla JM, Swamy MV, Rao CV. Diosgenin, a steroid saponin of Trigonella foenumgraecum (Fenugreek), inhibits azoxymethane-induced aberrant crypt foci formation in F344 rats and induces apoptosis in HT-29 human colon cancer cells. Cancer Epidemiology Biomarkers and Prevention. 2004;13(8):1392- 1398.

Francis G, Kerem Z, Makkar HP, Becker K. The biological action of saponins in animal systems: A review. British Journal of Nutrition. 2002;88(6):587-605.

Shabbeer S, Sobolewski M, Anchoori RK, Kachhap S, Hidalgo M, Jimeno A, Davidson N, Carducci MA, Khan SR. Fenugreek: A naturally occurring edible spice as an anticancer agent. Cancer Biology and Therapy. 2009;8(3):272- 278.
DOI: 10.4161/cbt.8.3.7443

Khoja KK, Shaf G, Hasan TN, Syed NA, Al-Khalifa AS, Al-Assaf AH, Alshatwi AA. Fenugreek, a naturally occurring edible spice, kills MCF-7 human breast cancer cells via an apoptotic pathway. Asian Pacific Journal of Cancer Prevention. 2011;12(12):3299-3304.

Tammanini C, Basini G, Grasselli F, Tirlli M. Nitric oxide and the ovary. American Society of Animal Science. 2003; 81(14_suppl_2): E1-E7,
DOI: 10.2527/2003.8114_suppl_2E1x.

Khare CP. Encyclopedia of Indian Medicinal Plants. Heidelberg: Springer-Verlag Berlin; 2004.

Kirk CJC, Pell RN, James KR, Lewis KS, Waft AD. Basic medical laboratory technology. 2nd ED. Pitman Books Ltd. London; 1982.

Kotb FT. Trigonella foenumgraecum. In Medicinal Plants in Libya. 1st Ed. Arab Encyclopedia House. 1985; 798-799.

McLean AC, Valenzuela N, Fai S, Bennett SAL. Performing Vaginal Lavage, Crystal Violet Staining and Vaginal Cytological Evaluation for Mouse Estrous Cycle Staging Identification. Journal of Visualized Experiments. 2012;15(67):e4389.
DOI: 10.3791/4389

Sulaiman IM, Nimir A, Ibrahim IA. Role of fenugreek extracts on vaginal cytology of female rats. Clinical and Experimental Medical Letters. 2011;52(1-2):13-18.

Junqueira LC, Carneiro J. Basic Histology. 10th Ed; 2003;449-467. Fenugreek Uses, Benefits & Side Effects [Online]; 2011.
[Accessed: 22 Feb 2017]

Baquer NZ, Kumar P, Taha A, Kale RK, Cowsik SM, McLean P. Metabolic and molecular action of Trigonella foenum-graecum (fenugreek) and trace metals in experimental diabetic tissues. Journal of Biosciences. 2011;36(2):383-396.

Modaresi M, Mahdian B, Jalalizand A. The Effect of Hydro-Alcoholic Extract of Fenugreek Seeds on Female Reproductive Hormones in Mice. International Conference on Applied Life Sciences; 2012.

Al-Oqail MM, Farshori NN, Al-Sheddi ES, Musarrat J, Al-Khedhairy AA, Siddiqui MA. In vitro cytotoxic activity of seed oil of fenugreek against various cancer cell lines. Asian Pacific Journal of Cancer Prevention. 2013;14(3):1829-1832.

Gupta A, Gupta R, Lal B. Effect of Trigonella foenum-graecum (fenugreek) seeds on glycaemic control and insulin resistance in type 2 diabetes mellitus: a double blind placebo controlled study. Journal of the Association of Physicians of India. 2001;49:1057–1061.

Chevassus H, Gaillard JB, Farret A, Costa F, Gabillaud I, Mas E, Dupuy AM, Michel F, Cantié C, Renard E, Galtier F, Petit P. A fenugreek seed extract selectively reduces spontaneous fat intake in overweight subjects. European Journal of Clinical Pharmacology. 2010;66(5):449-55, DOI: 10.1007/s00228-009-0770-0

Muraki E, Hayashi Y, Chiba H, Tsunoda N, Kasono K. Dose-dependent effects, safety and tolerability of fenugreek in diet-induced metabolic disorders in rat. Lipids in Health and Disease. 2011;10:240-246.

Naser A, ElSawy T, Ben Hadda EH, Bakr EAM Header AG, Fakim YN, Mabkhot M, Aljofan: (2014). Effects of crude aqueous extract of Origanum vulgaris in developing ovary of rabbits following in utero, adolescent and postpubertal exposure. VRI Phytomedicine. 2014;(3):77-84 e
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