Effects of Oral Administration of Red Palm Oil on Dichlorvos Induced Testicular Toxicity and Sperm Parameters in Albino Rats

Main Article Content

I. K. Lawal
A. D. Olasupo
A. A. Adekanmi
M. T. Afolabi
O. A. Owolabi
I. O. Aina

Abstract

Dichlorvos (2, 2-Dichlorovinyl Dimethyl Phosphate also known as DDVP) is an an organophosphate pesticide/ insecticide having oncogenic, genotoxic, neurotoxic and reproductive toxicity effects on the body. Elaeis guinensis (Palm oil) is an edible vegetable oil derived from the mesocarp ( reddish pulp) of fruit of the oil with antioxidant properties,  beta-carotene content with  highly saturated vegetable fats and is semi-solid at room temperature. This study was carried out to investigate the effects of oral administration of Red Palm Oil (RPO) on Dichlorvos (DDVP) induced testicular toxicity and sperm parameters in male albino rats. Concentration of 1ml of DDVP was diluted in 1000mls of distilled water and kept in a bottle for 3 days. 2 mls of red palm oil, 2 mls of red palm oil plus 1 ml of diluted DDVP, 1ml of diluted DDVP and 1 ml ordinary water were administered orally for 14 days respectively. Sperm parameters carried out were sperm count, motility, morphology and testicular histology analysis. The result obtained showed that DDVP administration significantly decrease sperm count, motility and morphological analysis (p<0.05). There is no significant difference in the value obtained for RPO induced rats in sperm count but significantly decrease in sperm motility and morphological analysis when compared with the value of the control (p<0.05). However, the group administered with RPO + DDVP shows significant increase in sperm count only. RPO histological analysis shows increase and well-formed seminiferous tubules with increase spermatogenic cells while DDVP shows laminar vacuolation, cellular degeneration and disruptions of seminiferous tubules. The group administered with RPO + DDVP shows an improving spermatogenic cells and normal seminiferous tubules. Therefore, this study establishes that RPO could offer positive protection against DDVP induced testicular damages.

Keywords:
Red palm oil, dichlorvos, testis, sperm count, motility and morphology.

Article Details

How to Cite
Lawal, I. K., Olasupo, A. D., Adekanmi, A. A., Afolabi, M. T., Owolabi, O. A., & Aina, I. O. (2021). Effects of Oral Administration of Red Palm Oil on Dichlorvos Induced Testicular Toxicity and Sperm Parameters in Albino Rats. Asian Journal of Research and Reports in Urology, 4(1), 13-19. Retrieved from http://journalajrru.com/index.php/AJRRU/article/view/30121
Section
Original Research Article

References

Obahiaghon. Review of the African oil and implications of its bioactive in human health; American Journal of Biochemistry and Molecular Biology; 2012.

Corley RHV, Tinker PBH. Assessment of growth, carbon stock and sequenstration potential of oil palm plantations in Mzoram, Northeast India. Journal of environmental protection. 2018;9(9).

Corley RHV. How much palm oil do we need? Environ. Sci. Policy. 2009;12:134–139.
DOI: 10.1016/j.envsci.2008.10.011

Tiemen Rhebergen, Shamie Zingore, Ken E Giller, Charles Adu Frimpong, Kwame Acheampant, Francis Ohipeni, Edward Kofi Payin. Closing yeild gap in oil palm production system in Ghana through best management practices: European Journal of Agronomy, 2020;115:126011.

Poku K. Small-scale palm oil processing in Africa. FAO agricultural services bulletin N°148, FAO, Roma; 2002.

Machini A, Imperlin E, Nigro E, Montagnese C, Daniele A, Orru S, Buono P. Biological and nutritional properties of Palm oil and palmitic acid: Effects on health, Molecules. 2015;20(9):17339-17361

Pantsi W, Marnewick J, Esterhuyse A, Rautenbach F, Van Rooyen J. Rooibos (Aspalathus linearis) offers cardiac protection against ischaemia/reperfusion in the isolated perfused rat heart. Phytomedicine. 2011;18(14):1220–1228.

Atawodi SE, Yusufu L, Atawodi JC, Asuku O, Yakubu OE. Phenolic compounds and antioxidant potential of Nigerian red palm oil (Elaeis guineensis) Int J Biol. 2011;3(2):153–161.

USEPA. United States Environmental Protection Agency. Dichlorvos TEACH Chemical summary U.S EPA. Toxicity and exposure assessment for children. 2007;1–13.

Owoeye O, Edem FV, Akinyoola BS, Rahaman S, Akang EE, Arinola GO. Toxicological changes in liver and lungs of rats exposed to dichlorvos before and after vitamin supplementation. Eur. J Anat. 2012;16(3):190–8.

WHO (1994–1995). World Health Organization. International Programme on chemical safety. UNEP/ILO/WHO. Recommended classification of pesticide by hazards and guidelines to classification; 1992.

Das S. A review of Dichlorvos toxicity in fish". Current World Environment Journal. 2013;8(1).

Wines M. Pesticide levels in waterways have dropped, reducing the risks to humans. The New York Times; 2014.

Hensaw Uchech, Okorowu Iwara, Arikpo Iwara. Dichlorvos toxicity: A public health perspective. Interdisciplinary Toxicology. 2018;12(2):129-132.

Booth ED, Jones E, Elliott BM. Review of the in vitro and in vivo genotoxicity of dichlorvos". Regulatory Toxicology and Pharmacology. 2007;49 (3): 316-26.

Faris SK. Effects of dichlorvos pesticide on fertility of laboratory male mice (Mus musculus L.) Bas J Vet Res. 2008;7(1):9–18.

Ezeji EU, Ogueri OD, Udebuani AC, Okereke JN, Kalu OO. Effects of Dichlorvos on the fertility of adult male albino rats. Nature and Science. 2015;13(12):1–5.

Jegede AI, Ajadi MB, Akinloye O. Modulatory effects of Kolaviron (Garcina kola extract) on spermogram and reproductive system of adult male Wistar rats in lead acetate induced toxicity,” Journal of Toxicology and Environmental Health Sciences. 2013;5(7):121–130.

Jegede AI, Offor U, Azu OO, Akinloye O. Red palm oil attenuates lead acetate induced testicular damage in adult male sprague-dawley rats. evidence-based complementary and Alternative Medicine. 2015;7.
Article ID 13026.

Nicholson DW. From bench to clinic with apoptosis-based therapeutic agents, Nature. 2000;407(6805):810–816.

Sundram K, Sambanthamurthi R, Tan YA. Palm fruit chemistry and nutrition, Asia Pacific Journal of Clinical Nutrition. 2003;12(3):355–362.

Aboua YG, Brooks N, Mahfouz RZ, Agarwal A, Du Plessis SS. A red palm oil diet can reduce the effects of oxidative stress on rat spermatozoa. Andrologia. 2012;44:32- 40.

Nangendran Balasundran, Tan Yew Ali, Ravigadevi Samban Thamuthi, Kalyana Sundran, Samir Samman. Antioxidant properties of palm fruit: Asia Pacific Journal of clinical nutrition. 2005;14(4):319-24.

Amaral S, Oliveira PJ, Ramalho-Santos J. Diabetes and the impairment of reproductive function: Possible role of mitochondria and reactive oxygen species. Curr Diabetes Rev. 2008;4(1):46-54.